Analysis of Salmonella typhimurium hisD3052 revertants: the use of oligodeoxyribonucleotide colony hybridization, PCR, and direct sequencing in mutational analysis.
Identifieur interne : 004890 ( Main/Exploration ); précédent : 004889; suivant : 004891Analysis of Salmonella typhimurium hisD3052 revertants: the use of oligodeoxyribonucleotide colony hybridization, PCR, and direct sequencing in mutational analysis.
Auteurs : E. Kupchella ; T A CebulaSource :
- Environmental and molecular mutagenesis [ 0893-6692 ] ; 1991.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Oligonucleotide Probes.
- chemical : Histidine.
- genetics : Salmonella typhimurium.
- Base Sequence, DNA Mutational Analysis, Molecular Sequence Data, Mutagenicity Tests, Nucleic Acid Hybridization, Polymerase Chain Reaction.
Abstract
A rapid method for determining the DNA sequences of Salmonella typhimurium hisD3052 revertants is presented. DNA colony hybridization was used to analyze revertants previously studied by Isono and Yourno [Proc Natl Acad Sci USA 71:1612-1617, 1974]. Synthetic oligodeoxyribonucleotide probes (18-mers) were able to distinguish sequences that differed by a single base pair. Mutant his sequences not identified by probing analysis were amplified using polymerase chain reaction (PCR) and directly sequenced. The combined use of DNA-colony hybridization and direct sequencing offers a precise and rapid means for the molecular characterization of hisD3052 revertants.
DOI: 10.1002/em.2850180404
PubMed: 1748083
Affiliations:
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DNA colony hybridization was used to analyze revertants previously studied by Isono and Yourno [Proc Natl Acad Sci USA 71:1612-1617, 1974]. Synthetic oligodeoxyribonucleotide probes (18-mers) were able to distinguish sequences that differed by a single base pair. Mutant his sequences not identified by probing analysis were amplified using polymerase chain reaction (PCR) and directly sequenced. The combined use of DNA-colony hybridization and direct sequencing offers a precise and rapid means for the molecular characterization of hisD3052 revertants.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Cebula, T A" sort="Cebula, T A" uniqKey="Cebula T" first="T A" last="Cebula">T A Cebula</name><name sortKey="Kupchella, E" sort="Kupchella, E" uniqKey="Kupchella E" first="E" last="Kupchella">E. Kupchella</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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